Our group has shown that numerous factors can influence how tissue engineered tendon constructs respond to in vitro mechanical stimulation. Although one study showed that stimulating mesenchymal stem cell (MSC)–collagen sponge constructs significantly increased construct linear stiffness and repair biomechanics, a second study showed no such effect when a collagen gel replaced the sponge. While these results suggest that scaffold material impacts the response of MSCs to mechanical stimulation, a well-designed intra-animal study was needed to directly compare the effects of type-I collagen gel versus type-I collagen sponge in regulating MSC response to a mechanical stimulus. Eight constructs from each cell line ( cell lines) were created in specially designed silicone dishes. Four constructs were created by seeding MSCs on a type-I bovine collagen sponge, and the other four were formed by seeding MSCs in a purified bovine collagen gel. In each dish, two cell-sponge and two cell-gel constructs from each line were then mechanically stimulated once every to a peak strain of 2.4%, for for 2 weeks. The other dish remained in an incubator without stimulation for 2 weeks. After 14 days, all constructs were failed to determine mechanical properties. Mechanical stimulation significantly improved the linear stiffness versus ; (standard error of the mean ) N/mm) and linear modulus ( versus ; MPa) of cell-sponge constructs. However, the same stimulus produced no such improvement in cell-gel construct properties. These results confirm that collagen sponge rather than collagen gel facilitates how cells respond to a mechanical stimulus and may be the scaffold of choice in mechanical stimulation studies to produce functional tissue engineered structures.
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e-mail: nirmalvs@email.uc.edu
e-mail: dressler@dordt.edu
e-mail: shearnj@email.uc.edu
e-mail: juncosln@email.uc.edu
e-mail: marepalli.rao@uc.edu
e-mail: goochc@uc.edu
e-mail: david.butler@uc.edu
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December 2007
Technical Briefs
Mechanical Stimulation of Tissue Engineered Tendon Constructs: Effect of Scaffold Materials
Victor S. Nirmalanandhan, Ph.D.,
Victor S. Nirmalanandhan, Ph.D.
Department of Biomedical Engineering,
e-mail: nirmalvs@email.uc.edu
University of Cincinnati
, 2901 Campus Drive, 837 Engineering Research Center, Cincinnati, OH 45221-0048
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Matthew R. Dressler, Ph.D.,
Matthew R. Dressler, Ph.D.
Department of Engineering,
e-mail: dressler@dordt.edu
Dordt College
, 498 4th Avenue, Northeast Sioux Center, IA 51250
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Jason T. Shearn, Ph.D.,
Jason T. Shearn, Ph.D.
Department of Biomedical Engineering,
e-mail: shearnj@email.uc.edu
University of Cincinnati
, Mail Location 0048, Cincinnati, OH 45221-0048
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Natalia Juncosa-Melvin, Ph.D.,
Natalia Juncosa-Melvin, Ph.D.
Department of Biomedical Engineering,
e-mail: juncosln@email.uc.edu
University of Cincinnati
, 2901 Campus Drive, 860 Engineering Research Center, Cincinnati, OH 45221-0048
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Marepalli Rao, Ph.D.,
Marepalli Rao, Ph.D.
Environmental Health-Genomics,
e-mail: marepalli.rao@uc.edu
University of Cincinnati
, Kettering 106, P.O. Box 670056, Cincinnati, OH 45267
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Cynthia Gooch, BS, LVT,
Cynthia Gooch, BS, LVT
Department of Biomedical Engineering,
e-mail: goochc@uc.edu
University of Cincinnati
, 2901 Campus Drive, 893 Engineering Research Center, Cincinnati, OH 45221-0048
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David L. Butler, Ph.D.
David L. Butler, Ph.D.
Professor
Director, Tissue Engineering and Biomechanics Laboratories, 840 Engineering Research Center, Department of Biomedical Engineering,
e-mail: david.butler@uc.edu
University of Cincinnati
, Cincinnati, OH 45221-0048
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Victor S. Nirmalanandhan, Ph.D.
Department of Biomedical Engineering,
University of Cincinnati
, 2901 Campus Drive, 837 Engineering Research Center, Cincinnati, OH 45221-0048e-mail: nirmalvs@email.uc.edu
Matthew R. Dressler, Ph.D.
Department of Engineering,
Dordt College
, 498 4th Avenue, Northeast Sioux Center, IA 51250e-mail: dressler@dordt.edu
Jason T. Shearn, Ph.D.
Department of Biomedical Engineering,
University of Cincinnati
, Mail Location 0048, Cincinnati, OH 45221-0048e-mail: shearnj@email.uc.edu
Natalia Juncosa-Melvin, Ph.D.
Department of Biomedical Engineering,
University of Cincinnati
, 2901 Campus Drive, 860 Engineering Research Center, Cincinnati, OH 45221-0048e-mail: juncosln@email.uc.edu
Marepalli Rao, Ph.D.
Environmental Health-Genomics,
University of Cincinnati
, Kettering 106, P.O. Box 670056, Cincinnati, OH 45267e-mail: marepalli.rao@uc.edu
Cynthia Gooch, BS, LVT
Department of Biomedical Engineering,
University of Cincinnati
, 2901 Campus Drive, 893 Engineering Research Center, Cincinnati, OH 45221-0048e-mail: goochc@uc.edu
Gino Bradica, Ph.D.
David L. Butler, Ph.D.
Professor
Director, Tissue Engineering and Biomechanics Laboratories, 840 Engineering Research Center, Department of Biomedical Engineering,
University of Cincinnati
, Cincinnati, OH 45221-0048e-mail: david.butler@uc.edu
J Biomech Eng. Dec 2007, 129(6): 919-923 (5 pages)
Published Online: April 24, 2007
Article history
Received:
August 1, 2006
Revised:
April 24, 2007
Citation
Nirmalanandhan, V. S., Dressler, M. R., Shearn, J. T., Juncosa-Melvin, N., Rao, M., Gooch, C., Bradica, G., and Butler, D. L. (April 24, 2007). "Mechanical Stimulation of Tissue Engineered Tendon Constructs: Effect of Scaffold Materials." ASME. J Biomech Eng. December 2007; 129(6): 919–923. https://doi.org/10.1115/1.2800828
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