Abstract
Deoxyribonucleic acid (DNA) was recovered from dried bloodstains aged up to three years and shown to be of high molecular weight. DNA was digested with restriction endonucleases and fractionated by agarose gel electrophoresis. Following transfer to a filter, DNA was hybridized with two different radioactively labeled recombinant probes which recognize highly polymorphic regions in human DNA. The autoradiographic pattern observed was not altered by sample age, and the size of the alleles was consistent with those observed in the general population. Therefore, DNA of high molecular weight prepared from dried blood samples can be used for identification.
Issue Section:
Research Papers
References
1.
Sensabaugh
, G. F.
, “Uses of Polymorphic Red Cell Enzymes in Forensic Science
,” Clinics in Haematology
, Vol. 10
, No. 1
, 02
1981
, pp. 185
-207
.2.
Sensabaugh
, G. F.
, “The Biochemistry of Individual Variation
,” Forensic Science Handbook
, Richard
Saferstein
, Ed., Prentice-Hall
, New York
, 1982
, pp. 339
-414
.3.
Kan
, Y. W.
and Dozy
, A. M.
, “Polymorphism of DNA Sequence Adjacent to the Human Betaglobin Structural Gene: Relationship to Sickle Mutation
,” Proceedings of the National Academy of Sciences (USA)
, Vol. 75
, No. 11
, 11
1978
, pp. 5631
-5635
.4.
Botstein
, D.
, White
, R. L.
, Skolnick
, M.
, and Davis
, R. W.
, “Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms
,” American Journal of Human Genetics
0002-9297, Vol. 32
, 05
1980
, pp. 314
-331
.5.
Maniatis
, T.
, Fritsch
, E. F.
, and Sambrook
, J.
, Molecular Cloning: A Laboratory Manual
, Cold Spring Harbor Laboratory
, Cold Spring Harbor, NY
, 1982
.6.
Rigby
, P. W. J.
, Dieckmann
, M.
, Rhodes
, C.
, and Berg
, P.
, “Labeling Deoxyribonucleic Acid to High Specific Activity in Vitro by Nick Translation with DNA Polymerase I
,” Journal of Molecular Biology
0022-2836, Vol. 113
, No. 1
, 15
06
1977
, pp. 237
-251
.7.
Vogelstein
, B.
and Gillespie
, D.
, “Preparative and Analytical Purification of DNA from Agarose
.” Proceedings of the National Academy of Sciences (USA)
, Vol. 76
, No. 2
, 02
1979
, pp. 615
-619
.8.
Wyman
, A. R.
and White
, R.
, “A Highly Polymorphic Locus in Human DNA
,” Proceedings of the National Academy of Sciences (USA)
, Vol. 77
, No. 11
, 11
1980
, pp. 6754
-6758
.9.
Capon
, D. J.
, Chen
, E. Y.
, Levinson
, A. D.
, Seeburg
, P. H.
, and Goeddel
, D. V.
, “Complete Nucleotide Sequences of the T24 Human Bladder Carcinoma Oncogene and Its Normal Homologue
,” Nature
0028-0836, Vol. 302
, No. 5903
, 03
03
1983
, pp. 33
-37
.10.
White
, R.
, Leppert
, M.
, Bishop
, D. T.
, Barker
, D.
, Berkowitz
, J.
, Brown
, C.
, Callahan
, P.
, Holm
, T.
, and Jerominski
, L.
, “Construction of Linkage Maps with DNA Markers for Human Chromosomes
,” Nature
0028-0836, Vol. 313
, No. 5998
, 10
01
1985
, pp. 101
-105
.11.
Nelson
, M. S.
, Turner
, L. L.
, and Reisner
, E. G.
, “A Feasibility Study of Human Leukocyte Antigen (HLA) Typing for Dried Bloodstains
,” Journal of Forensic Sciences
0022-1198, Vol. 28
, No. 3
, 07
1983
, pp. 608
-614
.12.
Denault
, G. C.
, Takimoto
, H. H.
, Kwan
, Q. Y.
, and Pallos
, A.
, “Detectability of Selected Genetic Markers in Dried Blood on Aging
,” Journal of Forensic Sciences
0022-1198, Vol. 25
, No. 3
, 07
1980
, pp. 479
-498
.
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