Abstract
Evidence material from sexual assault cases which had been submitted to the laboratory for routine processing were examined to determine the usefulness of the AmpliType® PM PCR Amplification and Typing Kit developed by Roche Molecular Systems for forensic evidence. In all cases in which a conclusive answer was reached for the AmpliType PM system, the results agreed with or surpassed results previously obtained with RFLP testing. The AmpliType PM DNA test system has promise as a quick and easy method for elimination or inclusion of suspects.
Issue Section:
Research Papers
References
1.
Giusti
, A.
, Baird
, M.
, Pasquale
, S.
, Balazs
, I.
, and Glassberg
, J.
, “Application of Deoxyribonucleic Acid (DNA) Polymorphisms to the Analysis of DNA Recovered from Sperm
,” Journal of Forensic Sciences
0022-1198, Vol. 31
, No. 2
, 04
1986
, pp. 409
-417
.2.
Budowle
, B.
and Baechtel
, F. S.
, “Modifications to Improve the Effectiveness of Restriction Fragment Length Polymorphism Typing
,” Applied and Theoretical Electrophoresis
0954-6642, Vol. 1
, 1990
, pp. 181
-187
.3.
Budowle
, B.
, Waye
, J. S.
, Schutler
, G. G.
, and Baechtel
, F. S.
, “HaeIII-A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples
,” Journal of Forensic Sciences
0022-1198, Vol. 35
, No. 3
, 05
1990
, pp. 530
-536
.4.
Saiki
, R. K.
, Scharf
, S.
, Faloona
, F.
, Mullis
, K. B.
, Horn
, G. T.
, Erlich
, H. A.
, and Arnheim
, N.
, “Enzymatic Amplification of β-globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia
,” Science
1095-9203, Vol. 230
, 1985
, pp. 1350
-1354
.5.
Saiki
, R. K.
, Gelfand
, D. H.
, Stoffel
, S.
, Scharf
, S. J.
, Higuchi
, R.
, Horn
, G. T.
, Mullis
, K. B.
, and Erlich
, H. A.
, “Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
,” Science
1095-9203, Vol. 239
, 1988
, pp. 487
-491
.6.
Helmuth
, R.
, Fildes
, N.
, Blake
, E.
, Luce
, M. C.
, Chimera
, J.
, Madej
, R.
, Gorodezky
, C.
, Stoneking
, M.
, Schmill
, N.
, Klitz
, W.
, Higuchi
, R.
, and Erlich
, H. A.
, “HLA-DQα Allele and Genotype Frequencies in Various Human Populations, Determined by Using Enzymatic Amplification and Oligonucleotide Probes
,” American Journal of Human Genetics
0002-9297, Vol. 47
, 1990
, pp. 515
-523
.7.
Saiki
, R. K.
, Walsh
, P. S.
, Levenson
, C. H.
, and Erlich
, H. A.
, “Genetic Analysis of Amplified DNA with Immobilized Sequence-Specific Oligonucleotide Probes
,” Proceedings of the National Academy of Sciences, USA
, Vol. 86
, 1989
, pp. 6230
-6234
.8.
Yamamoto
, T.
, Davis
, C. G.
, Brown
, M. S.
, Schneider
, W. J.
, Casey
, M. L.
, Goldstein
, J. L.
, and Russell
, D. W.
, “The Human LDL Receptor: A Cystein-Rich Protein with Multiple Alu Sequences in Its mRNA
,” Cell
0092-8674, Vol. 39
, 1984
, pp. 27
-38
.9.
Siebert
, P. D.
and Fukuda
, M.
, “Molecular Cloning of a Human Glycophorin B cDNA: Nucleotide Sequence and Genomic Relationship to Glycophorin A
,” Proceedings of the National Academy of Sciences, USA
, Vol. 84
, 1987
, pp. 6735
-6739
.10.
Slightom
, J. L.
, Blechl
, A. E.
, and Smithies
, O.
, “Human Fetal Gγ- and Aγ-Globin Genes: Complete Nucleotide Sequences Suggest That DNA Can Be Exchanged between These Duplicated Genes
,” Cell
0092-8674, Vol. 21
, 1980
, pp. 627
-638
.11.
Horn
, G. T.
, Richards
, B.
, Merrill
, J. J.
, and Klinger
, K. W.
, “Characterization and Rapid Diagnostic Analysis of DNA Polymorphisms Closely Linked to the Cystic Fibrosis Locus
,” Clinical Chemistry
0009-9147, Vol. 36
, 1990
, pp. 1614
-1619
.12.
Yang
, F.
, Brune
, J. L.
, Naylor
, S. L.
, Cupples
, R. L.
, Naberhaus
, K. H.
, and Bowman
, B. H.
, “Human Group-Specific Component (Gc) Is a Member of the Albumin Family
,” Proceedings of the National Academy of Sciences, USA
, Vol. 82
, 1985
, pp. 7994
-7998
.13.
Comey
, C. T.
and Budowle
, B.
, “Validation Studies on the Analysis of the HLA-DQα Locus Using the Polymerase Chain Reaction
,” Journal of Forensic Sciences
0022-1198, Vol. 36
, No. 6
, 11
1991
, pp. 1633
-1648
.14.
Comey
, C. T.
, Budowle
, B.
, Adams
, D. E.
, Baumstark
, A. L.
, Lindsey
, J. A.
, and Presley
, L. A.
, “PCR Amplification and Typing of the HLA-DQα Gene in Forensic Samples
,” Journal of Forensic Sciences
0022-1198, Vol. 38
, No. 2
, 03
1993
, pp. 239
-249
.15.
Blake
, E.
, Mihalovich
, J.
, Higuchi
, R.
, Walsh
, P. S.
, and Erlich
, H.
, “Polymerase Chain Reaction (PCR) Amplification and Human Leukocyte Antigen (HLA)-DQα Oligonucleotide Typing on Biological Evidence Samples: Casework Experience
,” Journal of Forensic Sciences
0022-1198, Vol. 37
, No. 3
, 05
1992
, pp. 700
-726
.16.
Walsh
, P. S.
, Metzger
, D. A.
, and Higuchi
, R.
, “Chelex 100 As a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material
,” BioTechniques
0736-6205, Vol. 10
, 1991
, pp. 506
-513
.17.
AmpliType® User Guide, Version 2,
Perkin-Elmer
, Norwalk, CT.18.
Waye
, J. S.
, Michaud
, D.
, Bowen
, J. H.
, and Fourney
, R. M.
, “Sensitive and Specific Quantification of Human Genomic Deoxyribonucleic Acid (DNA) in Forensic Science Specimens: Casework Examples
,” Journal of Forensic Sciences
0022-1198, Vol. 36
, No. 4
, 07
1991
, pp. 1198
-1203
.19.
Maniatis
, T.
, Fritsch
, E. F.
, and Sambrook
, J.
, Molecular Cloning: A Laboratory Manual
, 2nd ed., 1989
, Cold Spring Harbor Laboratory
, Cold Spring Harbor, NY
.
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