Abstract
In order to obtain fetal cells (e.g., for paternity cases) after abortion, the centrifugation of mountant (in our case formalin) may be tried when the DNA examination of the fixed tissue itself gives limited or no (or not enough) information. The fixed tissue was microscopically negative for fetal cells and gave no satisfactory results when examined for DNA. Centrifugation of approximately 50 mL of reddish colored formalin resulted in a cell pellet that was examined for DNA, which gave enough information to confirm a case of sexual abuse.
Issue Section:
Research Papers
References
1.
Ludes
BP
, Mangin
PD
, Malicier
DJ
, Chalumeau
AN
, Chaumont
AJ
. Parentage determination on aborted fetal material through deoxyribonucleic acid (DNA) profiling
. J Forensic Sci
0022-1198 1991
;36
:1219
–23
.2.
Wiegand
P
, Lorente
J
, Brinkmann
B
. DNA investigations on fetal material from paternity cases
. Int J Legal Med
0937-9827 1991
;104
:277
–80
.3.
Zehner
R
, Lasczkowski
G
. Paternity-testing on paraffin-embedded abortion tissue: preparation of fetal cells may be indispensable
. J Forensic Sci
0022-1198 2000
;45
:1332
–4
.4.
Old
JM
. Fetal DNA analysis
. In: Davies
KE
, ed. Human genetic disease analysis
. A practical approach
. 1993
; 2nd edition, IRL Press
, Oxford
New York
Tokyo
. 1
–19
.5.
Banaschak
S
, Sibbing
U
, Brinkmann
B
. Identifizierung von Gewebsproben bei Verdacht auf Materialvertauschung
. Pathologe
0172-8113 1997
;18
: 385–9.6.
Banaschak
S
, Rolf
B
, Brinkmann
B
. Influence of different staining techniques on the DNA analysis of histological sections
. Int J Legal Med
0937-9827 2000
;113
:114
–6
.7.
Wiegand
P
, Domhöver
J
, Brinkmann
B
. DNA-degradation in formalinfixiertem Gewebe
. Pathologe
0172-8113 1996
;17
:451
–4
.
This content is only available via PDF.
All rights reserved. This material may not be reproduced or copied, in whole or in part, in any printed, mechanical, electronic, film, or other distribution and storage media, without the written consent of ASTM International.
You do not currently have access to this content.