Abstract

Blood samples were obtained by venipuncture from random, consenting individuals of the three populations: Kongu Vellala Gounder (56), Irular (54), and Chakkiliyar (49). DNA was isolated using standard phenol/chloroform procedure (1). Fifteen autosomal microsatellite loci were amplified using the PowerPlex® 16 system (Promega Corporation, Madison) according to manufacturer's recommendations (2). The amplified products were separated by denaturing gel electrophoresis using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems, Foster City, CA). Alleles were determined by comparison with the allelic ladder included in the kit.

Issue Section:
Research Papers
Keywords:
forensic science, human identification, DNA profiling, short tandem repeats, D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1P0, Penta D, vWA, D8S1179, TPOX, FGA, population genetics, India, Tamil Nadu, Kongu Vellala Gounder, Irular, Chakkiliyar

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