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Technical Notes

A Simple DNA Extraction Method for Marijuana Samples Used in Amplified Fragment Length Polymorphism (AFLP) Analysis^*

HM Coyle, PhD,

HM Coyle, PhD

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

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G Shutler, PhD,

G Shutler, PhD

²Royal Canadian Mounted Police,

Forensic Laboratory Service-Winnipeg

,

Manitoba,

Canada

R3N 0E7

.

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S Abrams, BSc,

S Abrams, BSc

²Royal Canadian Mounted Police,

Forensic Laboratory Service-Winnipeg

,

Manitoba,

Canada

R3N 0E7

.

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J Hanniman, BSc (Hons),

J Hanniman, BSc (Hons)

²Royal Canadian Mounted Police,

Forensic Laboratory Service-Winnipeg

,

Manitoba,

Canada

R3N 0E7

.

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S Neylon, MS,

S Neylon, MS

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

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C Ladd, PhD,

C Ladd, PhD

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

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T Palmbach, Esq,

T Palmbach, Esq

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

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HC Lee, PhD

HC Lee, PhD

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

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Author and Article Information

HM Coyle, PhD

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

G Shutler, PhD

²Royal Canadian Mounted Police,

Forensic Laboratory Service-Winnipeg

,

Manitoba,

Canada

R3N 0E7

.

S Abrams, BSc

²Royal Canadian Mounted Police,

Forensic Laboratory Service-Winnipeg

,

Manitoba,

Canada

R3N 0E7

.

J Hanniman, BSc (Hons)

²Royal Canadian Mounted Police,

Forensic Laboratory Service-Winnipeg

,

Manitoba,

Canada

R3N 0E7

.

S Neylon, MS

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

C Ladd, PhD

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

T Palmbach, Esq

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

HC Lee, PhD

¹Division of Scientific Services,

Department of Public Safety

,

278 Colony Street, Meriden, CT

.

J. Forensic Sci.. Mar 2003, 48(2): 1-5 (5 pages)

https://doi.org/10.1520/JFS2001207

Published Online: March 1, 2003

Abstract

As a first step in developing a molecular method for the individualization of marijuana samples, we evaluated a plant DNA extraction kit. The QIAGEN plant DNeasy method uses a spin column format for recovery of DNA and is effective for obtaining high molecular weight DNA from leaf, flower (bud), and seed samples of marijuana. The average DNA yield was 125–500 ng per 100 milligrams of fresh plant tissue. The recovered DNA was of polymerase chain reaction (PCR) quality as measured by the ability to generate reproducible amplified fragment length polymorphism (AFLP) profiles. AFLP is a technique used to create a DNA profile for plant varieties and is being applied to marijuana samples by the authors to link growers and distributors of clonal material. The QIAGEN plant DNeasy method was simple, efficient, and reproducible for processing small quantities of marijuana into DNA.

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