Abstract

The samples for database studies were obtained from unrelated males from the indicated population groups residing in the state of Connecticut and were anonymized before analysis. The DNA from blood samples was extracted using the QIAamp® DNA blood MiniKit (Qiagen, Valencia, CA) following the recommended procedures. The quantity of human DNA was determined by slot blot hybridization using the QuantiBlot kit (Applied Biosystems, Foster City, CA) and following the manufacturer's recommended protocols. The Y-PLEX™6 system (ReliaGene Technologies, Inc., New Orleans, LA) was used for the amplification of the Y short tandem repeat (STR) loci. PCR amplification and the analysis of amplified product were performed as recommended by the manufacturer and as described in Sinha et al. (1).

Issue Section:
Editorial
Keywords:
forensic science, Y-chromosome, short tandem repeats, DNA typing, human identification, Y-STR, Y-PLEX, DYS393, DYS19, DYS389II, DYS390, DYS391,, DYS385

References

1.
Sinha
 
SK
,
Arcot
 
S
,
Richey
 
SL
,
Budowle
 
B
,
Chakraborty
 
R
,
Jones
 
MD
, et al.
Development and validation of a multiplexed Y-chromosome STR genotyping system, Y-PLEX™6, for forensic casework
.
J Forensic Sci
 0022-1198
2003
;
48
:in press.
2.
Schneider
 
PM
,
Meuser
 
S
,
Waiyawuth
 
W
,
Seo
 
Y
,
Rittner
 
C
.
Tandem repeat structure of the duplicated Y-chromosomal STR locus DYS385 and frequency studies in the German and three Asian populations
.
Forensic Sci Int
 0379-0738
1998
;
97
:
61
–
70
.
Google Scholar
Crossref
Search ADS
PubMed
 
3.
Roewer
 
L
,
Krawczak
 
M
,
Willuweit
 
S
,
Nagy
 
M
,
Alves
 
C
,
Amorim
 
A
, et al.
On line reference database of European Y-chromosomal short tandem repeat (STR) haplotypes
.
Forensic Sci Int
 0379-0738
2001
;
118
:
106
–
13
.
Google Scholar
Crossref
Search ADS
PubMed
 
4.
Redd
 
AJ
,
Clifford
 
SL
,
Stoneking
 
M
.
Multiplex DNA typing of shorttandem-repeat loci on the Y chromosome
.
Biol Chem
 1431-6730
1997
;
378
:
923
–
7
.
Google Scholar
PubMed
 
5.
Lessig
 
R
,
Edelmann
 
J
,
Krawczak
 
M
.
Population genetics of Y-chromosomal microsatellites in Baltic males
.
Forensic Sci Int
 0379-0738
2001
;
118
:
153
–
7
.
Google Scholar
Crossref
Search ADS
PubMed
 
This content is only available via PDF.
All rights reserved. This material may not be reproduced or copied, in whole or in part, in any printed, mechanical, electronic, film, or other distribution and storage media, without the written consent of ASTM International.
You do not currently have access to this content.