Abstract
DNA samples from 97 unrelated individuals were extracted by Chelex procedure (1) and then quantified using QuantiBlot® Human DNA Quantitation Kit according to the manufacturer's instructions (2). DNA samples (1 ng) were amplified and typed by Y-Plex™ 6 System (3). The electrophoresis was carried out on the ABI PRISM® 377 DNA Sequencer using GeneScan® y Genotyper® and Y-Typer Genotyping Software. Data were analyzed by B. Budowle according to Tajima (4) and Stoneking et al. (5). The complete data are available to any interested researcher by accessing http://www.cnea.gov.ar/cac/radiobiologia/yplex.xls.
Issue Section:
Editorial
Keywords:
forensic science,
DNA typing,
population genetics,
short tandem repeats,
DYS393,
DYS19,
DYS389,
DYS390,
DYS391,
DYS385
References
1.
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DA
, Higuchi
R
. Chelex 100 as a medium for simple extraction of DNA for PCR-based from forensic material
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.2.
Perkin Elmer
. QuantiBlot® Human DNA Quantitation Kit
, Revised ed., May, 1996.3.
Reliagene Corp
. Y-Plex™ 6
, Revised ed., March, 2001.4.
Tajima
F
. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism
. Genetics
0016-6731 1989
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–95
.5.
Stoneking
M
, Hedgecock
D
, Higuchi
RG
, Vigilant
L
, Erlich
HA
. Population variation of human mtDNA control region sequences detected by enzymatic amplification and sequence-specific oligonucleotide probes
. Am J Hum Genet
0002-9297 1991
;48
:370
–82
.
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