Abstract

Whole blood obtained by venipuncture from 107 unrelated males of the Han ethnic group in Chengdu, China. DNA was extracted using the Chelex method (1). The reaction volume of PCR was 37.5 μL, containing 2–4 ng human genome DNA, 200 μM each dNTP (Pharmacia, Sweden), 1.5μ Taq polymerase (Promega, Madison, WI), 3.75 μL 10 × buffer, Mg2+ 1.5 mM, 1.6 μg/mL BSA, 0.3 μM each primer. Amplification reactions were carried out in a Perkin Elmer 9600 (Foster City, CA) with pre-denaturing for 2 min at 94°C, followed by 35 cycles of denaturing for 40 s at 94°C, annealing for 40 s at 58°C and extension for 25 s at 72°C. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data of population genetics and forensic science were analyzed according to Hou's method (3).

References

1.
Walsh
BS
,
Petzger
DA
,
Higuchi
R
.
Chelex-100 as medium for simple extraction of DNA for PCR-based typing from forensic material
.
Biotechniques
 0736-6205
1991
,
10
:
506
10
.
2.
Allen
CR
,
Graves
G
,
Budowle
B
.
Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver
.
Biotechniques
 0736-6205
1990
;
7
:
736
44
.
3.
Hou
YP
,
Zhang
J
,
Li
YB
,
Wu
J
,
Zhang
S
,
Prinz
M
.
Allele sequences of six new Y-STR loci and haplotypes in the Chinese Han population
.
Forensic Sci Int
 0379-0738
2001
;
118
:
147
52
.
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