... DNA, 0.2 µM each primer, 10 mM Tris-HCl buffer (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 200 µM each dNTP, and 1 U AmpliTaq Gold® DNA polymerase (Applied Biosystems, Foster City, CA). DYS459 and DYS464 were co-amplified in a duplex reaction and DYS385 was amplified in a singleples reaction. Primer...

... tandem repeats haplotype DYS391 DYS389 I DYS439 DYS389 II DYS438 DYS437 DYS19 DYS392 DYS393 DYS390 DYS385 FOR THE RECORD J Forensic Sci, July 2005, Vol. 50, No. 4 Paper ID JFS2005006 Published 25 May 2005 Available online at: www.astm.org Lurdes Pontes,1 M.S.; Laura M. Caine´,1 M.S.; David...

...A Kido, PhD; Y Dobashi, MEng; R Susukida, MSc; N Fujitani, PhD; M Hara, PhD; M Oya, MD forensic science DNA typing population genetics Y-chromosome short tandem repeats haplotypes Indonesians DYS19 DYS385 DYS389II DYS390 DYS391 DYS393 DYS389I DYS392 DYS438 DYS439...

..., Y chromosome, short tandem repeat, Chinese ethnic group, allele frequency, DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385 We have analyzed the distribution of allele frequencies at seven Y-chromosomal short tandem repeat (STR) loci (DYS19, DYS389I/II, DYS390, DYS391, DYS392, DYS393...

...BW Cheng, Ph.D.; JX Xiao, Ph.D. 01 09 2004 forensic science DNA typing short tandem repeat polymerase chain reaction multiplex population genetics Yunnan Province DYS385 D10S676 References Schneider   PM , Meuser   S , Waiyawuth   W , Tandem repeat...

... bloodstains, the nonsperm fraction from a differentially extracted semen stain, and determination of the number of male donors in mixed semen stains. KEYWORDS: forensic science, SWGDAM validation, multiplex Y-STR analysis, post-coital cervicovaginal samples, DYS19, DYS385, DYS388, DYS389, DYS390, DYS391...

..., DYS393, DYS19, DYS390, DYS391, DYS385, DYS389I, DYS389II, DYS439, DYS438, DYS392, D2S1388, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, DQA1, FGA, GC, GYPA, HBGG, LDLR, Penta D, Penta E, TH01, TPOX, vWA Analysis for short tandem repeat loci (STRs) has...

... forensic science DNA typing fecal material polymerase chain reaction short tandem repeats DYS393 DYS19 DYS389II DYS390 DYS391 DYS385 DYS389I DYS439 DYS438 DYS392 D8S1179 D21S11 D7S820 CSF1PO D3S1358 TH01 D13S317 D16S539 D2S1338 D19S433 vWA TPOX D18S51 D5S818 FGA amelogenin...

...T Bhoopat, M.D.; C Hohoff, Dr. rer. nat.; HF Steger, Dr. rer. nat. Primers currently used for amplification of locus DYS385 on the Y chromosome give quite long amplicons that are difficult to resolve on native polyacrylamide gels. We therefore designed new primers that give much shorter products...

... and as described in Sinha et al. (1). forensic science Y-chromosome short tandem repeats DNA typing human identification Y-STR Y-PLEX DYS393 DYS19 DYS389II DYS390 DYS391, DYS385 References Sinha   SK , Arcot   S , Richey   SL , Budowle   B , Chakraborty   R , Jones   MD...

..., DYS389, DYS390, DYS391, DYS385 DNA samples from 97 unrelated individuals were extracted by Chelex procedure (1) and then quantified using QuantiBlot® Human DNA Quantitation Kit according to the manufacturer s instructions (2). DNA samples (1 ng) were amplified and typed by Y-Plex 6 System (3...

... for use in human identification. The Y-PLEX™6 kit enables simultaneous amplification of six polymorphic short tandem repeat (STR) loci located on the non-recombinant region of the human Y-chromosome. These loci are: DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385. Our studies show that as little...

... 12.5-1000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 amplified by using...

..., DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393, was carried out with a 25-ng template DNA in two multiplex reactions, standardized in the laboratory, using primers as described elsewhere (4,5). Amplified PCR products were run on a 5% polyacrylamide gel using a ABI 377 Automated DNA...

... loci DYS19, DYS389-II, DYS390, DYS391, DYS393 and, DYS385 were coamplified in a multiplex reaction using the Y-Plex6™ kit according to the manufacturers' recommendations (ReliaGene Technologies Inc.). The three others (DYS389-I, DYS392, and YCAII) were amplified by singleplex PCR. Primer sequences were...

.... (4). DYS385 locus was analyzed following the method and primers described by EDNAP group (5). Genotypes were analyzed in denaturing 6% polyacrilamide gel electrophoresis, using a monochrome automated laser fluorescence sequencer. The alleles from all loci reported were typed according...

...S Jung, Ph.D.; D Patzelt, M.D. Whole liquid blood was obtained from unrelated individuals. DNA extraction was performed using a salting out procedure (1). PCR was carried out for D3S1358, D8S1179, D18S51 (2), D21S11 (3), and DYS385 (4) with 5 ng of DNA each. The PCR products were separated using...

... M.D.; Barbara Boninfante,3 B.Sc.; Ginevra Guanti,2 M.D.; Grazia Forleo,2 Nicoletta Resta,2 B.Sc. Distribution of DYS391, DYS392, DYS393, DYS385, Alleles in a Southern Italian Population Sample POPULATION: Southern Italian (from Apulia); N 73 KEYWORDS: forensic science, Y short tandem repeats...

...-specific STRs was calculated to be 98.94%. Comparison of allelic frequencies of the nine loci except the DYS385 locus between the previous data for the Japanese populations (3,4) using the X2-test showed significant differences in their distributions except DYS393. In addition, comparison of the patterns...

...J-J Gamero, Ph.D.; J-L Romero, Ph.D.; J-L González, Ph.D.; M Carvalho, B.S.; M-J Anjos, B.S.; F Corte-Real, Ph.D.; D-N Vieira, Ph.D.; M-C Vide, B.S. A Population-Genetic Study of the DYS385 Haplotypes in Two Spanish Populations and the African Immigrant Population in Spain forensic science...