...), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 µL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had...

... variation at the three Y-STR loci named as DYS434, DYS443, and DYS456, were analyzed by PCR amplication with multiplex system. Multiplex PCR amplifications were performed in a 37.5 μL containing 2–4 ng human genome DNA, 7.5 μL dNTP (1 mmol/mL), 3μ, Taq polymerase, 3.75 μL 10 × buffer (Mg 2+ 1.5 mmol/l...