Electrophoretic systems commonly use metal electrodes in their construction. This paper explores and reports the differences in the electrophoretic motion of DNA (decomposition voltage, electrical field, etc.) when one electrode is constructed from a semiconductor, silicon, rather than metal. Experimental (voltage-current) curves for different electrode configurations (using steel and silicon) are presented. Experimental results are used to update and validate the mathematical model to reflect the differences in material selection. In addition, the model predicts large curved-field motion for DNA motion. The model helps to quantify the effect of parameters on DNA motion in biological microelectromechanical systems in order to improve device designs and protocols.
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Research Papers
Effects of Dissimilar Electrode Materials and Electrode Position on DNA Motion During Electrophoresis
Regis A. David,
Regis A. David
Department of Mechanical Engineering,
Brigham Young University
, Provo, UT 84602
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Brian D. Jensen,
Brian D. Jensen
Department of Mechanical Engineering,
e-mail: bdjensen@byu.edu
Brigham Young University
, Provo, UT 84602
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Justin L. Black,
Justin L. Black
Department of Microbiology and Molecular Biology,
Brigham Young University
, Provo, UT 84602
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Sandra H. Burnett,
Sandra H. Burnett
Department of Microbiology and Molecular Biology,
Brigham Young University
, Provo, UT 84602
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Larry L. Howell
Larry L. Howell
Department of Mechanical Engineering,
Brigham Young University
, Provo, UT 84602
Search for other works by this author on:
Regis A. David
Department of Mechanical Engineering,
Brigham Young University
, Provo, UT 84602
Brian D. Jensen
Department of Mechanical Engineering,
Brigham Young University
, Provo, UT 84602e-mail: bdjensen@byu.edu
Justin L. Black
Department of Microbiology and Molecular Biology,
Brigham Young University
, Provo, UT 84602
Sandra H. Burnett
Department of Microbiology and Molecular Biology,
Brigham Young University
, Provo, UT 84602
Larry L. Howell
Department of Mechanical Engineering,
Brigham Young University
, Provo, UT 84602J. Nanotechnol. Eng. Med. May 2011, 2(2): 021014 (6 pages)
Published Online: May 17, 2011
Article history
Received:
January 7, 2011
Revised:
January 27, 2011
Online:
May 17, 2011
Published:
May 17, 2011
Citation
David, R. A., Jensen, B. D., Black, J. L., Burnett, S. H., and Howell, L. L. (May 17, 2011). "Effects of Dissimilar Electrode Materials and Electrode Position on DNA Motion During Electrophoresis." ASME. J. Nanotechnol. Eng. Med. May 2011; 2(2): 021014. https://doi.org/10.1115/1.4003600
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